cdna fragment for mturqoise2 Search Results


jeremy purvis  (ATCC)
96
ATCC jeremy purvis
Jeremy Purvis, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+fragment+for+mturqoise2/pm34800361-191-21-16?v=ATCC
Average 96 stars, based on 1 article reviews
jeremy purvis - by Bioz Stars, 2026-08
96/100 stars
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cdna fragment for mturqoise2  (Addgene inc)
94
Addgene inc cdna fragment for mturqoise2
Cdna Fragment For Mturqoise2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+fragment+for+mturqoise2/bio_rxiv__2024__06__29__601303-43-1-5?v=Addgene+inc
Average 94 stars, based on 1 article reviews
cdna fragment for mturqoise2 - by Bioz Stars, 2026-08
94/100 stars
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b4galt1 mturqoise2 mturqoise golgi  (Addgene inc)
94
Addgene inc b4galt1 mturqoise2 mturqoise golgi
(A–B) INS-1E β-cells were transfected with wild-type proinsulin (PI) labeled with mCherry or eGFP and with the autolysosome markers LAMP-1-eGFP or with P62/SQSTM1-mCherry. After 24 h, cells were treated with bafilomycin-A1 for different times, followed by confocal microscope imaging. (B) Quantification of proinsulin-P62/SQSTM1 colocalization (n=3). (C–D) INS-1ECells were transfected with proinsulin-eGFP or proinsulin-mCherry and P62/SQSTM1-mCherry and then treated with 50nmol/l rapamycin for 16 h. (D) Quantification of proinsulin-P62/SQSTM1 colocalization (lower panel) (n=3). (E) Cells were incubated with cycloheximide for 2 h with or without 50nmol/l rapamycin and proinsulin content analyzed by ELISA (n=3 in triplicates). (F) INS-1E cells were treated overnight with 100mmol/l trehalose or with tat-Beclin1 for 3 h. Autophagy was assessed by Western blotting for LC3-II and P62/SQSTM1 and proinsulin content analyzed by ELISA (n=3 in triplicates). (G) INS-1E cells were transfected with proinsulin-eGFP, LC3-mCherry and <t>B4GALT1-mTurquoise2</t> (mTurquoise-Golgi) constructs, then treated with and without bafilomycin A1. Proinsulin and <t>B4GALT1</t> localization in autophagosomes (LC3-eGFP+ punctae) analyzed by confocal microscope. Size bar=10 μm.
B4galt1 Mturqoise2 Mturqoise Golgi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+fragment+for+mturqoise2/pmc05912938-110-40-44?v=Addgene+inc
Average 94 stars, based on 1 article reviews
b4galt1 mturqoise2 mturqoise golgi - by Bioz Stars, 2026-08
94/100 stars
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aav plasmids coding for mturqoise2 only (mt), mturqoise2-p2a-hvp16 (h-16), and mturqoise2-p2a-pvp16 (p-16)  (GenScript corporation)
90
GenScript corporation aav plasmids coding for mturqoise2 only (mt), mturqoise2-p2a-hvp16 (h-16), and mturqoise2-p2a-pvp16 (p-16)
(A–B) INS-1E β-cells were transfected with wild-type proinsulin (PI) labeled with mCherry or eGFP and with the autolysosome markers LAMP-1-eGFP or with P62/SQSTM1-mCherry. After 24 h, cells were treated with bafilomycin-A1 for different times, followed by confocal microscope imaging. (B) Quantification of proinsulin-P62/SQSTM1 colocalization (n=3). (C–D) INS-1ECells were transfected with proinsulin-eGFP or proinsulin-mCherry and P62/SQSTM1-mCherry and then treated with 50nmol/l rapamycin for 16 h. (D) Quantification of proinsulin-P62/SQSTM1 colocalization (lower panel) (n=3). (E) Cells were incubated with cycloheximide for 2 h with or without 50nmol/l rapamycin and proinsulin content analyzed by ELISA (n=3 in triplicates). (F) INS-1E cells were treated overnight with 100mmol/l trehalose or with tat-Beclin1 for 3 h. Autophagy was assessed by Western blotting for LC3-II and P62/SQSTM1 and proinsulin content analyzed by ELISA (n=3 in triplicates). (G) INS-1E cells were transfected with proinsulin-eGFP, LC3-mCherry and <t>B4GALT1-mTurquoise2</t> (mTurquoise-Golgi) constructs, then treated with and without bafilomycin A1. Proinsulin and <t>B4GALT1</t> localization in autophagosomes (LC3-eGFP+ punctae) analyzed by confocal microscope. Size bar=10 μm.
Aav Plasmids Coding For Mturqoise2 Only (Mt), Mturqoise2 P2a Hvp16 (H 16), And Mturqoise2 P2a Pvp16 (P 16), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+fragment+for+mturqoise2/pm38869285-79-7-15?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
aav plasmids coding for mturqoise2 only (mt), mturqoise2-p2a-hvp16 (h-16), and mturqoise2-p2a-pvp16 (p-16) - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


(A–B) INS-1E β-cells were transfected with wild-type proinsulin (PI) labeled with mCherry or eGFP and with the autolysosome markers LAMP-1-eGFP or with P62/SQSTM1-mCherry. After 24 h, cells were treated with bafilomycin-A1 for different times, followed by confocal microscope imaging. (B) Quantification of proinsulin-P62/SQSTM1 colocalization (n=3). (C–D) INS-1ECells were transfected with proinsulin-eGFP or proinsulin-mCherry and P62/SQSTM1-mCherry and then treated with 50nmol/l rapamycin for 16 h. (D) Quantification of proinsulin-P62/SQSTM1 colocalization (lower panel) (n=3). (E) Cells were incubated with cycloheximide for 2 h with or without 50nmol/l rapamycin and proinsulin content analyzed by ELISA (n=3 in triplicates). (F) INS-1E cells were treated overnight with 100mmol/l trehalose or with tat-Beclin1 for 3 h. Autophagy was assessed by Western blotting for LC3-II and P62/SQSTM1 and proinsulin content analyzed by ELISA (n=3 in triplicates). (G) INS-1E cells were transfected with proinsulin-eGFP, LC3-mCherry and B4GALT1-mTurquoise2 (mTurquoise-Golgi) constructs, then treated with and without bafilomycin A1. Proinsulin and B4GALT1 localization in autophagosomes (LC3-eGFP+ punctae) analyzed by confocal microscope. Size bar=10 μm.

Journal: Diabetologia

Article Title: Autophagy is a major regulator of β-cell insulin homeostasis

doi: 10.1007/s00125-016-3868-9

Figure Lengend Snippet: (A–B) INS-1E β-cells were transfected with wild-type proinsulin (PI) labeled with mCherry or eGFP and with the autolysosome markers LAMP-1-eGFP or with P62/SQSTM1-mCherry. After 24 h, cells were treated with bafilomycin-A1 for different times, followed by confocal microscope imaging. (B) Quantification of proinsulin-P62/SQSTM1 colocalization (n=3). (C–D) INS-1ECells were transfected with proinsulin-eGFP or proinsulin-mCherry and P62/SQSTM1-mCherry and then treated with 50nmol/l rapamycin for 16 h. (D) Quantification of proinsulin-P62/SQSTM1 colocalization (lower panel) (n=3). (E) Cells were incubated with cycloheximide for 2 h with or without 50nmol/l rapamycin and proinsulin content analyzed by ELISA (n=3 in triplicates). (F) INS-1E cells were treated overnight with 100mmol/l trehalose or with tat-Beclin1 for 3 h. Autophagy was assessed by Western blotting for LC3-II and P62/SQSTM1 and proinsulin content analyzed by ELISA (n=3 in triplicates). (G) INS-1E cells were transfected with proinsulin-eGFP, LC3-mCherry and B4GALT1-mTurquoise2 (mTurquoise-Golgi) constructs, then treated with and without bafilomycin A1. Proinsulin and B4GALT1 localization in autophagosomes (LC3-eGFP+ punctae) analyzed by confocal microscope. Size bar=10 μm.

Article Snippet: Cells were seeded on 8-chamber borosilicate coverglass plates (Nunc, Roskilde, Denmark) and transfected the subsequent day with the following constructs using Lipofectamine-2000 and Optimem (Life Technologies, Carlsblad, CA): LAMP1-eGFP [ 17 ], LC3-mCherry [ 17 ], P62/SQSTM1-mCherry [ 18 ], B4GALT1-mTurqoise2 (mTurqoise-Golgi) (plasmid #36205; Addgene) [ 19 ], WT proinsulin-eGFP, WT proinsulin-mCherry, Akita proinsulin-eGFP [ 20 ] and G(B23)V proinsulin-eGFP [ 21 ]. eGFP was excited using a 488nm argon laser and mCherry with a 561nm helium/neon laser.

Techniques: Transfection, Labeling, Microscopy, Imaging, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Construct